Fig. 4

Acupuncture reduced P2X4 and ox42 expression in DPN rats. a qRT-PCR showing relative mRNA level of P2X4 and OX42 on different days post-acupuncture. b Representative western blot of P2X4 and OX42 protein levels on 1 and 14 days post-acupuncture. c Representative photomicrographs of immunofluorescence of P2X4 (green) and OX42 (red) at the beginning (1 d) and at the end of acupuncture treatment (14 d) (Original magnification × 400) and its fluorescence intensity. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control, ^#P < 0.05, ^###P < 0.001 vs. DPN